Construcción de cepas de Escherichia coli a partir de minicélulas por transferencia lateral de genes

Recently, artificial life is created with artificial materials and methods. Life can be born, when genomic DNA molecule integrated in liposomes containing biochemical reactions for biogenic needs. However, it is not yet known whether the integration of these parts will be able to occur in nature, and constitute a living system. I planned to regenerate bacteria from biologically active liposomes by inserting genomic DNA using all natural materials and methods. The mini-cells of Escherichia coli, containing plasmids and activated SOS proteins, act as protocells. Four new E. coli strains were regenerated from mini-cells by inserting the genomes with the conjugation system between F^- and Hfr strains. Four regenerated cells showed the same genetic markers as two genome donors. The pulse field gel electrophoresis of their genomes showed admixing of those of both two donors. In addition, the genome of 4 regenerated cells had chimera genome of two donors. Our results represent synthesis of life can occur in nature without artificial arrangement.

IMPORTANCE What is the difference between inanimate objects and organisms? Organisms have genomic DNA by all means. When organisms lose their genome, they cannot neither grow nor reproduce. As the result, organisms turn into inanimate objects without their genome. In this manuscript, I regenerated microbes from the cell corpse that lost its genome by inserting another genome. All steps of regeneration applied natural behavior of microbes. The same regeneration of microbes would happen in nature. The primitive lives have plasticity, which accelerates evolution and provides various kinds of lives in the world.

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